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Highly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dNTPs undergo highly stringent purification steps to give a greater than 99% purity and are tested or the absence of DNase, RNase, Protease, Nickase activity. They are then quality controlled& in a variety of applications, ensuring that they are highly suitable for the most sensitive of PCR techniques. All Bioline dNTP Mixes are supplied as an equimolar solution of ultra-pure dATP, dCTP, dGTP and dTTP as lithium salts. Lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life. As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for nucleotides are beyond the scope of our standard product range, Bioline can also offer custom, bulk and OEM services (firstname.lastname@example.org).
Product Highlights Excellent clarity - enhances visualization, even at high concentrations Strong gels at low concentration - better handling, less breakage DNase/RNase-free - for all nucleic acid separation Product Description Extremely pure, high molecular biology grade Agarose from Bioline has no detectable DNase or RNase activity and forms strong gels with low backgrounds. Due to its low EEO, DNA will have a high electrophoretic mobility.
Product Highlights Mixture of oligonucleotides -for RNA missing a polyA tail such as bacterial RNA Binds randomly -for RNA with strong secondary structure Product Description Random Hexamer Primers consist of a mixture of oligonucleotides representing all possible hexamer sequences. Random Hexamer Primers are commonly used for priming single-stranded DNA or RNA for extension by DNA polymerases or reverse transcriptases. During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between mRNA and other RNA species present in the reaction. Primer Sequence: 5´ – d (NNNNNN) –3´ N = G, A, T or C A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
Product Highlights Long oligonucleotide -more thermostable Reverse Transcriptases perform better with longer primers Binds only to mRNA -can be used for cDNA synthesis with total RNA Product Description Oligo (dT)18 Primer is suitable for use as a primer for first strand cDNA synthesis with a reverse transcriptase. The primer hybridizes to the poly-adenylated tail found on the 3´ end of most eukaryotic mRNAs. Oligo (dT)18 ensures that the 3´ end of mRNAs are represented. Primer sequence: 5´-d (TTT TTT TTT TTT TTT TTT)-3´ A mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.
RiboSafe RNase Inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C. Product Highlights Efficient – effectively inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C High-quality - certified free of contaminating DNases, RNases, Phosphatases and Nickases for improved sample security Robust – active up to 55 oC across a wide pH range and DTT concentrations, ideal for use most downstream applications Flexible – compatible with all common reverse transcriptases, bacterial polymerases and thermostable polymerases Product Description RiboSafe RNase Inhibitor is a recombinant protein which completely inhibits a broad spectrum of eukaryotic RNases, including RNases A, B and C. Furthermore, RiboSafe shows no inhibition of polymerase or reverse transcriptase activity and so can be used for cDNA synthesis and in one-step RT-PCR reactions. RiboSafe RNase Inhibitor is tested for activity, SDS-PAGE purity and the absence of endonucleases, nickases and exonucleases and functions robustly at a variety of temperatures and pH and in the presence or absence of DTT, allowing flexibility in designing experiments in highly-sensitive techniques such as single-cell RT-PCR, in vitro RNA synthesis and in vitro translation.
Product Highlights Reduced recombination of cloned DNA - designed to accommodates larger plasmids High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning Easy screening - with blue/white color selection Convenient size - small aliquots reduce waste Product Description α-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.
Product Highlights Induces E.coli lac operon activity - suitable for cloning and protein expression procedures Dioxane free - does not disrupt normal cell function >99.6% purity - confirmed by HPLC Convenient - available as powder and stabilized stock solution Product Description Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.
Product Highlights Perfect for cloning - gives an intense blue precipitate upon hydrolysis by β-galactosidase Extremely pure - confirmed by HPLC Product Description 5-bromo-4-chloro-3-indolyl ß-D-galactopyranoside (X-GAL) is a chromogenic substrate for ß-Galactosidase that forms an intense blue precipitate.It can be used in molecular biology to detect the gal gene product, and also in microbiology where it is used to detect micro-organisms which have ß-Galactosidase activity (usually coliforms). It can be combined with the R-substrates to differentiate between two species of organisms on the same plate. X-GAL is soluble in N, N-dimethylformamide. X-GAL is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.
Fully autoclavable, Ergonomic design maximizing user comfort, Easy-to-read volume display, Easy calibration and maintenance,
A powerful mix 8-channel with 96-well plate, Dispensing head rotating for effortless pipetting, Individual piston and tip cone assemblies allow easy repair and maintenance, Compound material-made tip cone securing high sealing performance Compatible with most universal tip bands,
A powerful mix 12-channel with 96-well plate, Dispensing head rotating for effortless pipetting, Individual piston and tip cone assemblies allow easy repair and maintenance, Compound material-made tip cone securing high sealing performance Compatible with most universal tip bands,
Appropriate for most of the plastic and glass pipettes from 0.1-100ml, Eight speeds of selection for aspiration and dispensing different liquids, Large LCD Display showing low battery warning and speed settings, Enabling single-handed operation with minimun effort, High capacity Li-on battery enable long runtime of operation, 0.45um replaceable Hydrophobic filter.
Enabling single-handed operation with minimun effort, Appropriate for most of the plastic and glass from 0.1-100ml, Easily adjusted speed control, 0.45um replaceable Hydrophobic filter, Stable and reliable preformance, elegant apperance, High Capacity Li-on battery enable long runtime of operation
Ease of use, precise pipetting control, Robust and lightweight, Suitable for 0.1-100ml volumetric or serological pipettes, Filling and delivery comfortably achieved with a sensitive lever, for integral or replacement filter, 3um, easy cleaning and maintenancen, autocalvable silicone pipette holder.
Light and ergonomic solution provide easy usability, Single-handed operation, Volume range fron 1ul to 5ml, Up to 48 dispensing steps, Maintenance free, Equipped with durable tip insertion lever, Fits seven sizes of disposable polypropylene syringes from 0.5ml to 50ml.
High quality motor providing excellent precision and accuracy, Electronic control decreases the repetitive strain injury, the remove control panel makes the operating process without the manual disturbance, Build-in magnetic stirrer support.
Motor operation minimizes the grip force, Electronic control decreases the repetitive strain injury, the remote control panel makes the operating process without the manual disturbance, Two disoensing modes, dispenser and stepper function options.
Excellent chemical resistance, components are made of (PTFE, FEP, BSG, PP), Fully autoclavable at 121C, Four ranges of bottle-top dispenser covering a volume range from 0.5ml to 50ml, Easy for cleaning and maintenance, the Optional flexible discharge tube with safety handle permits fast and precise dispensing, Dispensemate is supplied with S40, GL32, GL38, GL25, GL28 sized adaptors.