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RT007 AMV. reverse transcriptase was isolated from Avian Myeloblastosis Virus ( AMV) as the holoenzyme of molecular weight 157,000 Daltons, using a modification of the method described by Houts etc. This preparation is free of nuclease. It is qualified for cDNA synthesis and also for dideoxy sequencing of DNA and RNA Total RNA or poly A+ RNA can be used as template, and optimal temperature is 42-55'C, up to 60°C. If NaPPi Is used, 37~41'C is preferred. RT reaction buffer covered by patents can be used either as 5X /10X stock depending on different purpose (details in standard application). Up to 10-12kb cDNA may be obtained according to standard application.
Biospin Plant Genomic ONA Extraction Kit provides a very simple, fast and economic way to isolate pure high molecular-weight genomic DNA from plant tissues. The simple purification procedure, based on the remarl<able selectivity of Biospin membrane, allows isolation of high yields of pure genomic DNA less than 40min.lt doesn't require any expensive equipment, or toxic reagents. Generally, 1-30µg genomrc DNA can be acquired from up to 100 mg plant tissue. The pure DNA can be applied extensively rn PCR/Real-trme PCR, sequencing, Southern blot, mutant analysis, SNP and the others.
Due to the corrosive nature of the phenol, preparation of phenol solution for use in a molecular biology laboratory is a time-consuming and often hazardous pr<?cedure. Frequentty it is necessary to redistill the phenol prior to use to remove contaminants and oxidation products that can damage nucleic acids. In some cases, the PH phenol solutions needs to be adjusted before use. Safety precautions such as protective eyewear, gloves, and a fume hood are necessary when you use the product.
Red Blood Cell Lysis Buffer was developed to lyse red blood cells of human whole blood. The buffer provides a very simple, fast economic way to isolate white blood cells from whole blood without destroying the white blood cells. In general, with the buffer, you can get 80% or more white blood cells from the sample.
This product is the original water through reverse osmosis technology and EDI technology to remove the heavy metals, microorganisms, organic matter, endotoxin and other impurities. Then purify by Milli-O pure waler machine.Can be used rn HPLC, LC, GC/MS, ICP-MS, ILC, PCR, two--d1mensional electrophoresis, tissue culture and molecular biology and others.