ช็อปตามแบรนด์
ISOLATE II RNA/DNA/Protein Kit, 50 preps

The ISOLATE II RNA/DNA/Protein Kit provides a simple, efficient column-based method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidine-thiocyanate lysis with the speed and ease-of-use of three silica-membrane purification columns, the ISOLATE II RNA/DNA/Protein Kit provides a fast method for the purification of high-quality total RNA, genomic DNA and proteins from a single sample of cultured cells, mammalian tissue, biofluids (including saliva, urine, semen and blood), bacteria, yeast, fungi or plants. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit.  The ISOLATE II RNA/DNA/Protein Kit allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The purified nucleic acids and proteins are of the highest purity and integrity and the ISOLATE II RNA/DNA/Protein Kit has been designed to deliver optimal performance in a wide variety of downstream applications including miRNA profiling using the EPIK Panel and Select Assays, novel biomarker discovery, siRNA gene silencing studies, gene expression profiling, as well as epigenetics, genotyping, transgenic analysis and cell line characterization.

34,000 บาท
53,700 บาท -37%
ISOLATE II Biofluids RNA Kit, 50 preps

The ISOLATE II Biofluid RNA Kit provides a simple, efficient column-based method for the isolation of total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Biofluid RNA Kit provides a fast method for the purification of high-quality total RNA from Biofluids including blood, serum, plasma, urine, saliva and cerebrospinal fluid. A genomic DNA removal column is used to remove contaminating genomic DNA, however for applications that are very sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II Biofluid RNA Kit has been designed to deliver optimal performance in RT-qPCR in conjunction with either the SensiFAST cDNA Synthesis Kit and SensiFAST Real-Time PCR Kits, or the SensiFAST One-Step Real-Time RT-PCR Kits. Additionally, the ISOLATE II Biofluid RNA Kit can be used to purify samples prior to PCR and RT-PCR amplification using the Tetro cDNA Synthesis Kit and any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.

13,500 บาท
21,900 บาท -38%
MyTaq™ Plant-PCR Kit, 250RXN

MyTaq™ Plant PCR Kit is recommended for fast, specific and direct PCR from a wide range of plant leaf samples. MyTaq Plant-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors. MyTaq Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. The advanced formulation of MyTaq Plant-PCR Kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield. MyTaq Plant-PCR Kit has been developed to tolerate the PCR inhibitors typically present in plant samples, including polyphenolics and polysaccharides, thereby delivering significantly improved assay sensitivity and reproducibility. MyTaq Plant-PCR Kit increases amplification success rates from different plant types and its speed and high specificity makes it ideal for high-throughput genotyping assays. Bioline R&D has obtained successful results when using the MyTaq Plant-PCR Kit to conduct direct PCR from a very broad range of species. These include tomato, rice, sugarcane, maize, potato, soya, wheat, barley, cashew, oak, dwarf umbrella tree, Australian laurel, red-fruit saw-sedge, blueberry lily, she-oaks, coastal rosemary, burrawang, eucalyptus/gum tree, seaweed/crayweed, coastal banksia, bottle brush and honey gem. For more information, please inquire. As part of our test and review campaign, many of our customers have shared their positive experiences of the MyTaq Plant-PCR Kit. Our customers have also reported success for direct PCR from wheat, rosemary, cotton, tobacco, citrus, kangkong, mousear cress (Arabidopsis), cranberry, primrose, blueberry, American bellflower and several grass and fungus species.

12,900 บาท
18,200 บาท -29%
ISOLATE II miRNA Kit, 25 preps

The ISOLATE II miRNA Kit provides a simple, efficient column-based method for the isolation of miRNA and total RNA from a wide variety of starting materials, without the need for hazardous reagents such as phenol, which has been reported to introduce significant bias in recovery of selected small RNAs. By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of two silica-membrane purification columns, the ISOLATE II miRNA Kit provides a fast method for the purification of high-quality total RNA (on the first column) and miRNA (on the second column) from cultured animal cells, small tissue samples, bacterial cells, plants and blood. For applications that are sensitive to very small amounts of DNA, residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment with RNase-free DNase I that is supplied with the kit. The ISOLATE II miRNA Kit has been designed to deliver optimal performance with the EPIK Panel and Select Assays in miRNA expression profiling and quantification using qPCR, microarrays, small RNA library construction for small RNA-Seq, as well as single cell assays.

12,600 บาท
18,200 บาท -31%
ISOLATE II Plant DNA Kit, 50 preps

The ISOLATE II Plant DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of plant materials, without the need for hazardous reagents such as phenol. By combining the stringency of CTAB lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plant DNA Kit provides a fast method for the purification of high-quality genomic DNA from most plant cells, particularly those rich in polysaccharides, including leaves, bark, roots and fruits as well as dung, animal-fecal, soil and compost samples. Some plant tissues do not lyse well in CTAB and so an SDS-based lysis buffer is also provided as an alternative. Residual amounts of RNA remaining can be removed using an RNase treatment during lysis with RNase A that is supplied with the kit.  The ISOLATE II Plant DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR with any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase                                     "Isolating DNA from soil samples is a big challenge because samples are full of inhibitors like polymers or low-molecular substances, which inhibit downstream applications. Therefore, it is difficult to find a kit which can deliver stable DNA. The ISOLATE II Plant DNA Kit is able to handle these kinds of samples and worked well for soil samples." Katharina Schulte, Technical College Zweibruecken, Germany

8,900 บาท
13,500 บาท -34%
ISOLATE II Genomic DNA Kit, 50 preps

The ISOLATE II Genomic DNA Kit provides a simple, efficient column-based method for the isolation of genomic DNA from a wide variety of materials, without the need for hazardous reagents such as phenol. By combining Proteinase K lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Genomic DNA Kit provides a fast method for the purification of high-quality genomic DNA from a variety of starting materials, such as mouse or rat tails, bacteria, yeast, dried blood spots, genomic/viral DNA from blood, hair follicles, paraffin-embedded tissue (FFPE), stool viruses (e.g. CMV), Mycobacterium tuberculosis or Legionella pneumophila in sputum or bronchoalveolar lavage, EHEC bacteria in food, bacterial DNA (e.g. Chlamydia trachomatis) from cultures, biological fluids or clinical specimens, bacterial DNA (e.g. Borrelia burgdorferi) and viral DNA (e.g. CMV) from urine, insects, dental swabs and buccal swabs. The ISOLATE II Genomic DNA Kit has been designed to deliver optimal performance in qPCR in tandem with the SensiFAST Real-Time PCR Kits and in end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase.                   "I used the ISOLATE II Genomic DNA Kit to extract genomic DNA from mice tails for genotyping. The kit comes with very clear, user friendly instructions and provides efficient, reliable and cost effective isolation of DNA. Overall a great kit for genomic DNA extraction and downstream analysis." Wilson Wong, University of Sydney, Camperdown, Australia

6,500 บาท
9,900 บาท -34%
SensiFAST SYBR Hi-ROX Kit, 500RXN

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Sensitive – reliable quantification of low abundance targets and scarce samples Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 30 minutes Product Description The SensiFAST™ SYBR Hi-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX. A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Hi-ROX Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.  The SensiFAST SYBR® Hi-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

4,900 บาท
7,700 บาท -36%
SensiFAST SYBR Lo-ROX Kit, 500RXN

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Sensitive – reliable quantification of low abundance targets and scarce samples Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 30 minutes Product Description The SensiFAST™ SYBR Lo-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR Lo-ROX Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.  The SensiFAST SYBR® Lo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

4,900 บาท
7,700 บาท -36%
SensiFAST SYBR No-ROX Kit, 500RXN

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Sensitive – reliable quantification of low abundance targets and scarce samples Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 30 minutes Product Description The SensiFAST™ SYBR No-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions.  An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.  The SensiFAST SYBR® No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

4,900 บาท
7,700 บาท -36%
SensiFAST Probe Lo-ROX Kit, 500RXN

Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions. Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Sensitive – reliable quantification of low abundance targets and scarce samples Fast – delivers reproducible, accurate assay results in as little as 30 minutes Efficient – excellent performance in multiplex assays Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Product Description The SensiFAST™ Probe Lo-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays. The SensiFAST Probe Lo-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

4,900 บาท
7,700 บาท -36%
SensiFAST Probe No-ROX Kit, 500RXN

Product Highlights Reproducible – consistent results between technical replicates for increased confidence in results Robust – reliable, accurate detection of DNA and RNA targets from a broad range of sample types Sensitive – reliable quantification of low abundance targets and scarce samples Fast – delivers reproducible, accurate assay results in as little as 30 minutes Efficient – excellent performance in multiplex assays Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Product Description The SensiFAST™ Probe No-ROX Kit has been developed for fast, highly reproducible real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. A combination of the latest advances in buffer chemistry and PCR enhancers, together with an antibody-mediated hot-start DNA polymerase, ensures that the SensiFAST Probe Kit produces reliable assay results under fast thermal cycling conditions. Furthermore, SensiFAST Probe has been optimized to deliver excellent results in multiplex assays. The SensiFAST Probe No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

4,900 บาท
7,700 บาท -36%
IPTG, 5 g

Product Highlights Induces E.coli lac operon activity - suitable for cloning and protein expression procedures Dioxane free - does not disrupt normal cell function >99.6% purity - confirmed by HPLC Convenient - available as powder and stabilized stock solution Product Description Isopropyl-ß-D-thiogalactopyranoside (IPTG) is a chemical analogue of galactose, which cannot be hydrolyzed by the enzyme ß--Galactosidase. Hence, it induces the E. coli lac operon activity by binding and inhibiting the lac repressor without being degraded. Genes controlled by the lac or tac promotor/operator sequences are expressed to high levels in the presence of IPTG. IPTG is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.

4,900 บาท
6,800 บาท -28%
ISOLATE II PCR and Gel Kit, 50 preps

The ISOLATE II PCR and Gel Kit provides a simple, efficient membrane-based method for the purification of DNA from PCR reactions and from TAE and TBE agarose gels, without the need for hazardous reagents or ethanol precipitation. Six PCR products can be purified in 10 minutes using simple binding and elution steps. Concentrated PCR products ranging between 60 bp and 15 kb can be eluted, removing primers, nucleotides, enzymes, mineral oil, salts and other impurities. DNA fragments between 50 bp and 20 kb can be extracted from six agarose gel slices in about 20 minutes using a color indicator to help maintain optimal pH and identify undissolved agarose. The ISOLATE II PCR and Gel Kit has been designed to deliver optimal performance in transformations, cloning, sequencing and restriction analysis.

4,600 บาท
7,200 บาท -36%
X-GAL, 1 g

Product Highlights Perfect for cloning - gives an intense blue precipitate upon hydrolysis by β-galactosidase Extremely pure - confirmed by HPLC Product Description 5-bromo-4-chloro-3-indolyl ß-D-galactopyranoside (X-GAL) is a chromogenic substrate for ß-Galactosidase that forms an intense blue precipitate.It can be used in molecular biology to detect the gal gene product, and also in microbiology where it is used to detect micro-organisms which have ß-Galactosidase activity (usually coliforms). It can be combined with the R-substrates to differentiate between two species of organisms on the same plate. X-GAL is soluble in N, N-dimethylformamide. X-GAL is part of the Bioline Essentials range of products, which includes agaroses, buffers, PCR water and enhancers, antibiotic solutions, cloning reagents and protein digestion reagents.

4,600 บาท
6,200 บาท -26%
Alpha-Select Bronze Efficiency

Product Highlights Reduced recombination of cloned DNA - designed to accommodates larger plasmids High efficiency - available in ≥107 cfu/μg, perfect for routine subcloning Easy screening - with blue/white color selection Convenient size - small aliquots reduce waste Product Description α-Select Bronze Competent Cells are directly comparable to DH5α cells. They contain a lacZ marker that provides α-complementation of the ß-galactosidase gene for blue/white color screening and are ideal for subcloning using plasmid-derived vectors. α-Select Bronze Competent Cells also provide recA1 and endA1 markers to minimize recombination and enhance the quality of the plasmid DNA, allowing efficient transformation even with large plasmids.

4,500 บาท
6,000 บาท -25%
ISOLATE II Plasmid Mini Kit, 50 preps

The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol. By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II Plasmid Mini Kit provides a fast method for the purification of high-quality plasmid DNA. Separate protocols are provided for the isolation of high-copy plasmids, the isolation of low-copy plasmid, P1 constructs and cosmid DNA from E. coli and the isolation of plasmids from gram-positive bacteria such as Bacillus, Staphylococcus, Bifidobacteria or Corynebacteria. The ISOLATE II Plasmid Mini Kit has been designed to deliver optimal performance in cloning, sequencing as well as end-point PCR alongside any enzyme from the Bioline PCR portfolio, including MyTaq DNA Polymerase. Additionally, the ISOLATE II Plasmid Mini Kit can be used in tandem with the SensiFAST Real-Time PCR Kits for high-performance qPCR.

4,500 บาท
5,800 บาท -22%
HyperPAGE II, 25-50 lane

HyperPAGE II is a broad range prestained protein marker containing nine highly purified recombinant proteins with molecular weights ranging from 10kDa to 250kDa. The proteins are prestained with several fluorescent dyes and resolve into sharp, clearly identifiable bands for easy visualization and orientation. The green 10kDa, orange 70kDa and pink 140kDa bands serve as useful reference bands. HyperPAGE II is suitable to monitor migration efficiency in SDS-PAGE and protein transfer from gel to membrane in Western blotting, without the addition of staining dye. Prestained protein markers are suitable for determining the approximate molecular weights of proteins.   

4,300 บาท
7,600 บาท -43%
HyperLadder™ 50bp, 100 lane

HyperLadder™ 50bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels. The 10 regularly spaced bands, ranging from 50 bp to 2000 bp, with higher intensity reference bands at 300 bp, 1000 bp and 2000 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 50bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 50bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 50bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 50bp is perfect for size determination in techniques such as confirmation of PCR products as well as other downstream techniques.

2,900 บาท
4,500 บาท -36%
HyperLadder™ 25bp, 100 lane

HyperLadder™ 25bp is a molecular weight marker, especially designed for easy size determination of linear double-stranded DNA fragments on 2% to 3% TAE or TBE agarose gels or polyacrylamide gels. The 10 regularly spaced bands, ranging from 25 bp to 500 bp, with higher intensity reference bands at 100 bp and 200 bp allow for easy identification and orientation of your band at a glance. This ready-to-use format reduces handling steps and saves time; simply transfer HyperLadder 25bp from the vial to the gel. The concentration of DNA in each of the HyperLadder 25bp bands is provided for optionally determining the approximate mass of DNA in comparably intense sample bands of similar size. The HyperLadder 25bp is also supplied with a 5x loading dye for loading of sample DNA. HyperLadder 25bp is perfect for size determination in techniques that produce very short fragments such as apoptotic DNA fragmentation as well as other downstream techniques.

2,900 บาท
4,000 บาท -28%
HYPERLADDER 100 BP PLUS, 100 LANES

2,300 บาท
3,500 บาท -34%
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