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Tetro cDNA Synthesis Kit, 100RXN

The Tetro cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other applications, such as analyses of cellular RNAs, characterization of RNA splice variants and the generation and cloning of cDNA.  The Tetro cDNA Synthesis Kit is optimized for RT reactions over a wide range of total RNA concentrations (10 pg-2 μg), such that long and low-abundance cDNAs can be detected by amplification after cDNA synthesis. The kit contains oligo (dT)18 and random hexamer primers. The kit components are fully optimized to generate maximum yields of full-length cDNA.

9,500 บาท
12,500 บาท -24%
SensiFAST cDNA Synthesis Kit, 50RXN

To complement the SensiFAST™ Probe and SYBR® kits, Bioline has developed the SensiFAST™ cDNA Synthesis Kit to provide a rapid and sensitive method for first-strand cDNA synthesis. The SensiFAST™ cDNA Synthesis Kit displays excellent linearity across a wide range of starting material, revealing the same relative representation in cDNA templates, regardless of gene abundance, making it excellent for use in real-time PCR studies. A novel, highly-pure reverse transcriptase and new TransAmp™ buffer system delivers highly-efficient synthesis of cDNA, enhanced reproducibility and data accuracy. These features make the SensiFAST™ cDNA Synthesis Kit ideal for working with limited samples, such as laser-micro dissected samples and tissue biopsies. The TransAmp Buffer also employs a unique blend of random hexamer primers and anchored oligo dT to ensure unbiased 3’ and 5’ coverage and reverse transcription of all regions. Additionally, the SensiFAST™ cDNA Synthesis Kit can be used with SensiFAST™ Probe and SYBR® Kits for fast real-time RT-PCR without compromising quality, with real-time results in less than an hour.  "Our Institute consisting of more than 60 active researchers has a longstanding interest in understanding changes to the transcriptional landscape of prostate cancer. Reproducibility, sensitivity and cost-effectiveness are critical parameters in selecting our day-to-day cDNA synthesis kit. This combined with its convenient two reagent system continues to make the SensiFast cDNA synthesis kit stand out from its competitors and our preference for almost two years." Martin Sadowski, Queensland University of Technology, Brisbane, Australia

7,700 บาท
11,000 บาท -30%
SensiFAST Probe No-ROX One-Step Kit, 100RXN

The SensiFAST Probe No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST™ Probe No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.                                                     "I compared the SensiFAST Probe One-Step Kit with another very popular product. SensiFAST Probe One-Step gave improved sensitivity, which was reflected in lower Ct values. Also, this product is a very good price. Value for money!!" Peony Fung, Cerberus Sciences, Thebarton, Australia

5,500 บาท
7,300 บาท -25%
SensiFAST Probe Lo-ROX One-Step Kit, 100RXN

The SensiFAST Probe Lo-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST™ Probe Lo-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes. "I compared the SensiFAST Probe One-Step Kit with another very popular product. SensiFAST Probe One-Step gave improved sensitivity, which was reflected in lower Ct values. Also, this product is a very good price. Value for money!!" Peony Fung, Cerberus Sciences, Thebarton, Australia

5,500 บาท
7,300 บาท -25%
SensiFAST SYBR Lo-ROX One-Step Kit, 100RXN

Product Highlights Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Robust – reliable detection of RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 40 minutes                                                           The SensiFAST SYBR® Lo-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST SYBR® Lo-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a low concentration of the passive reference dye ROX.

5,100 บาท
8,000 บาท -36%
SensiFAST SYBR No-ROX One-Step Kit , 100RXN

Product Highlights Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Robust – reliable detection of RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 40 minutes                                                         The SensiFAST™ SYBR® No-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation. The SensiFAST SYBR® No-ROX One-Step Kit has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.

5,100 บาท
7,300 บาท -30%
SensiFAST SYBR HI-ROX One-Step Kit , 100RXN

Product Highlights Sensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targets Reproducible – consistent results between technical replicates for increased confidence in results Specific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliability Robust – reliable detection of RNA targets from a broad range of sample types Fast – delivers reproducible, accurate assay results in as little as 40 minutes                                                         The SensiFAST™ SYBR® Hi-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube.  An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.  The SensiFAST SYBR® Hi-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX. 

5,100 บาท
7,300 บาท -30%
MyTaq ONE-STEP RT-PCR Kit, 100RXN

MyTaq™ One-Step RT-PCR Kit has been designed for extremely sensitive and highly reproducible first-stand cDNA synthesis and subsequent PCR in a single tube. The kit contains the latest advances in buffer chemistry, including Bioline ultra-pure dNTPs, together with a proprietary reverse transcriptase and MyTaq HS DNA Polymerase which is our next generation of very high performance, antibody-mediated hot-start DNA polymerase. This ensures that MyTaq One- Step RT-PCR Kit produces fast, highly-specific and ultra-sensitive products for downstream applications. MyTaq One-Step Kit consists of reverse transcriptase, 2x MyTaq HS Mix and the potent RNase Inhibitor RiboSafe, that are blended to create a simple to use all-in-one mix. The kit is ideal for determining the presence or absence of RNA templates and quantifying expression through qualitative, semi-quantitative or quantitative analysis of RNA transcription levels, and the one-step format is also perfect for the synthesis of double stranded cDNA products for subsequent gene expression analysis. The cDNA can be synthesized with starting amounts of RNA template from 3 pg to 1 μg, over a broad temperature range, and up to 50°C to overcome secondary structure and GC-rich sequences), prior to heating to 95°C to inactivate reverse transcriptase and simultaneously to activate the MyTaq™ HS. "When we compared the performance of our routine supplier’s RTase against Bioline’s MyTaq One-Step RT-PCR, the other supplier’s RTase gave two false negatives in five different grapevine samples tested for Grapevine rupestris stem-pitting-associated Foveavirus. We were convinced to immediately switch." University of Adelaide, Australia

4,900 บาท
6,500 บาท -25%
TRIsure, 100 ml

TRIsure provides a simple, efficient method for the sequential isolation of total RNA, genomic DNA and proteins from a wide variety of starting materials. By combining thiocyanate compounds with phenol, TRIsure facilitates disruption of cells during homogenization and effectively inhibits DNase and RNase activity. After homogenizing the sample with TRIsure, chloroform is added and the homogenate is allowed to separate into three phases: a clear aqueous phase (upper) containing the RNA, a green colored organic phase (lower) and an interphase, containing the DNA and proteins. The RNA is extracted from the aqueous phase by isopropyl alcohol precipitation, DNA is precipitated from the organic layer with ethanol and the proteins are precipitated from the phenol-ethanol supernatant by isopropyl alcohol precipitation. The precipitated RNA, DNA, or protein is washed to remove impurities and then resuspended for use in downstream applications. TRIsure allows for highly reliable integrative analysis of the transcriptome, genome and proteome, since the RNA, DNA and proteins are derived from the same sample and the same cell population. The simplicity of the TRIsure method allows simultaneous processing of a large number of samples and can be used in a wide variety of downstream applications including expression profiling, epigenetics, genotyping, transgenic analysis and cell line characterization.

4,400 บาท
7,000 บาท -37%
MyTaq HS RED DNA Polymerase, 1000 units

MyTaq™ HS Red DNA Polymerase is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. The MyTaq HS DNA Polymerase and MyTaq Red Reaction Buffer in this product, are a unique combination of next-generation hot-start DNA polymerase and novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme, specifically designed for highly-specific, efficient amplification from even the most challenging templates. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. Furthermore, the advanced formulation of MyTaq HS and buffer system allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield. The enzyme is supplied with a 5x MyTaq Red Reaction Buffer that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq Red Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

4,400 บาท
5,500 บาท -20%
TETRO REVERSE TRANSCRIPTASE, 10000U

Tetro Reverse Transcriptase is a Moloney Murine Leukaemia Virus (MMLV) Reverse Transcriptase, which exhibits high stability, with no loss of activity following 1 week at room temperature. Tetro Reverse Transcriptase is highly sensitive even when the amount of template is a limiting factor (fig. 1), with highly efficient and sensitive transcription, from as little as 10 pg, up to 2 μg of RNA (fig. 2). Many RNA transcripts form stable secondary structures at lower temperatures, making them less suitable as templates for RT-PCR at those temperatures. Tetro Reverse Transcriptase is suitable for first-strand cDNA synthesis, with total RNA, mRNA and in vitro transcribed RNA and shows excellent performance with gene-specific primers, Oligo (dT) as well as random hexamers, making it perfect for cDNA library construction and the production of templates for RT-PCR analysis of gene expression.

4,200 บาท
5,500 บาท -24%
MyTaq HS Red Mix, 2x, 200RXN

MyTaq HS Red Mix is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS Red Mix is comprised of MyTaq HS DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq HS has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors.  Furthermore, the highly efficient nature of MyTaq HS means it gives excellent results under fast PCR conditions. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. MyTaq Red Mix contains a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, MyTaq Red contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

3,600 บาท
4,300 บาท -16%
Proteinase K (Powder), 100mg

Proteinase K is a highly active serine protease (MW 28,500 Da) isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of native RNA and DNA from tissues or cell lines. Because the solution is tested for the absence of RNases and DNases, it is ideal for isolating PCR and RT-PCR templates. The activity of Proteinase K is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 μg/mL for protein removal and enzyme inactivation and up to 2 mg/mL for tissue treatment. Proteinase K products are free of detectable DNase and RNase.

3,500 บาท
5,900 บาท -41%
Agarose, Molecular Grade, 100 g

Product Highlights Excellent clarity - enhances visualization, even at high concentrations Strong gels at low concentration - better handling, less breakage DNase/RNase-free - for all nucleic acid separation Product Description Extremely pure, high molecular biology grade Agarose from Bioline has no detectable DNase or RNase activity and forms strong gels with low backgrounds. Due to its low EEO, DNA will have a high electrophoretic mobility.

3,400 บาท
6,000 บาท -43%
Ranger DNA Polymerase, 250 units

RANGER DNA Polymerase is recommended for all Long PCR applications. RANGER is an easy-to-use, high performance enzyme, specifically developed to amplify fragments up to 25 kb in length. RANGER contains a unique combination of a high-performance DNA polymerase and novel buffer system that deliver the improved efficiency necessary for reliable amplification of longer amplicons. RANGER is an antibody-mediated hot-start enzyme that eliminates non-specific amplification during reaction set-up. The inactivated enzyme does not possess polymerase activity, thereby preventing the non-specific amplification, such as primer-dimer formation, that often hinder Long PCR reactions from the start. RANGER is provided with a specially formulated reaction buffer containing dNTPs, MgCl2 and enhancers at optimal concentrations, minimizing the requirements for PCR optimization, in turn reducing time to results and eliminating the cost of unnecessary repeats.

3,200 บาท
5,000 บาท -36%
MyFi DNA Polymerase, 250 units

MyFi™ has been developed to give reliable amplification of targets up to 10 kb from challenging and complex targets, even in the presence of PCR inhibitors. MyFi is therefore ideal for amplification of cDNA libraries, complex genomic fragments and GC-rich targets. Additionally, MyFi shows improved tolerance to PCR inhibitors, thereby enabling reliable detection from samples from which DNA is difficult to purify. Furthermore, its unique buffering system and enzyme blend promote highly sensitive amplification of even very low-copy number targets. The proofreading ability of MyFi allows all PCR products to be cloned. The inclusion of MyTaq HS means MyFi generates PCR products with 3’-A overhangs, which is perfect for TA cloning. MyFi has the added convenience of room temperature reaction assembly, to avoid non-specific amplification and primer-dimer formation.

3,200 บาท
4,500 บาท -29%
MyTaq Red Mix, 2x, 200RXN

MyTaq™ Red Mix is recommended for all standard PCR applications. MyTaq Red Mix is comprised of MyTaq DNA Polymerase and a novel buffer system that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases allowing it to perform well with challenging templates and in the presence of PCR inhibitors. Furthermore, the highly efficient nature of MyTaq means it gives excellent results under fast PCR conditions. The product is supplied as a mastermix that requires the addition of only template, primers and water, thereby reducing the number of pipetting steps during PCR set-up, for improved speed, throughput and assay reproducibility. MyTaq Red Mix contains a red dye that increases the visual contrast between the reagent and the reaction vessel for improved convenience and to improve pipetting accuracy. The red dye also enables samples to be loaded directly on to a gel after the PCR without the need to add loading buffer. In addition, the MyTaq red reaction buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

2,500 บาท
4,200 บาท -40%
VELOCITY DNA Polymerase, 250 units

VELOCITY DNA Polymerase is recommended for high-fidelity PCR amplification. The enzyme possesses a 3’ – 5’ proofreading exonuclease activity that provides an exceptional error rate of 4.4 x 10-7 for highly accurate amplification from a very broad range of human, animal and plant targets. Furthermore, VELOCITY is a highly processive enzyme with extension rates as fast as 15 s/kb for targets up to 5 kb and 30 s/kb for targets up to 10 kb, thereby enabling a reduction in PCR turnaround times. VELOCITY delivers exceptional fidelity with outstanding PCR yield even from low template concentrations. The increased processivity of VELOCITY, results in shorter extension times for fast PCR, increased product yield and the ability to amplify longer fragments. VELOCITY also offers robust and reliable product yields, even in assays where PCR conditions are challenging, including the presence of impurities or GC-rich targets. VELOCITY is a high-performance DNA polymerase, ideally suited for high-yield, fast PCR amplification of even long targets containing no mutations.

2,500 บาท
3,600 บาท -31%
IMMOLASE DNA Polymerase , 500 units

IMMOLASE™ is a heat-activated thermostable DNA polymerase and provides high yield (fig. 1) and improved specificity when compared to standard polymerases. IMMOLASE can eliminate the presence of non-specifics such as primer-dimers and mis-primed products. IMMOLASE is inactive at room temperature and requires activation by heat treatment for 10 minutes prior to PCR cycling (fig. 2). This provides greater flexibility in reaction set ups and allows the premixing of PCR reagents at room temperature. Subsequently, the reaction can be handled according to preferred protocols for thermostable DNA polymerases.

2,500 บาท
3,100 บาท -19%
MyTaq™ HS DNA Polymerase, 500 units

MyTaq™ HS DNA Polymerase is recommended for PCR assays containing complex and low copy number targets as well as multiplex PCR. MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme, specifically designed for highly-specific, efficient amplification from even the most challenging templates. MyTaq HS does not possess polymerase activity during the reaction set-up, thereby reducing the non-specific amplification that can hinder PCR assays from the start. The MyTaq HS DNA Polymerase and MyTaq Reaction Buffer in this product are a unique combination of next-generation hot-start polymerase and novel buffer system, that deliver very high yield PCR amplification over a wide range of PCR templates. MyTaq HS has an increased affinity for DNA, enabling reliable amplification from even very low amounts of template. MyTaq& HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases, meaning it performs reliably even in the presence of PCR inhibitors. Furthermore, the advanced formulation of MyTaq HS and buffer system allows fast cycling conditions, considerably reducing the reaction time without compromising PCR specificity or yield. MyTaq Reaction Buffer contains dNTPs, MgCl2 and enhancers at optimal concentrations, which helps eliminate the need for optimization, thereby saving time, effort and the cost of performing unnecessary assay repeats.

2,200 บาท
3,400 บาท -35%
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